| 56137 |
Creator |
c3baa1aae2495d9fcf383c433b46bfe0 |
| 56137 |
Creator |
ext-05301bdb209eb94604b67b58a785e68b |
| 56137 |
Creator |
ext-2d050b20bbc92b58be9fe508b66fb5e3 |
| 56137 |
Creator |
ext-797c0d82a822a7888c27fc4222f66c07 |
| 56137 |
Creator |
ext-ed2af8554a2fbd3ad6cba245f4a6469f |
| 56137 |
Creator |
ext-f71612a445dcd800f42b95d2461a8dd6 |
| 56137 |
Creator |
ext-fd41cb56e27c816cba4055fbed55aeee |
| 56137 |
Date |
2019-02 |
| 56137 |
Is Part Of |
repository |
| 56137 |
Is Part Of |
p17485460 |
| 56137 |
abstract |
<b>Objective</b><br></br>To modify the non-porous surface membrane of a tissue-engineered
laryngeal scaffold to allow effective cell entry.<br></br><b>Methods</b><br></br>The
mechanical properties, surface topography and chemistry of polyhedral oligomeric silsesquioxane
poly(carbonate-urea) urethane were characterised. A laser technique introduced surface
perforations. Micro computed tomography generated porosity data. Scaffolds were seeded
with cells, investigated histologically and proliferation studied. Incubation and
time effects were assessed.<br></br><b>Results</b><br></br>Laser cutting perforated
the polymer, connecting the substructure with the ex-scaffold environment and increasing
porosity (porous, non-perforated = 87.9 per cent; porous, laser-perforated at intensities
3 = 96.4 per cent and 6 = 89.5 per cent). Cellular studies confirmed improved cell
viability. Histology showed cells adherent to the scaffold surface and cells within
perforations, and indicated that cells migrated into the scaffolds. After 15 days
of incubation, scanning electron microscopy revealed an 11 per cent reduction in pore
diameter, correlating with a decrease in Young's modulus.<br></br><b>Conclusion</b><br></br>Introducing
surface perforations presents a viable method of improving polyhedral oligomeric silsesquioxane
poly(carbonate-urea) urethane as a tissue-engineered scaffold. |
| 56137 |
authorList |
authors |
| 56137 |
issue |
2 |
| 56137 |
status |
peerReviewed |
| 56137 |
uri |
http://data.open.ac.uk/oro/document/657901 |
| 56137 |
uri |
http://data.open.ac.uk/oro/document/657902 |
| 56137 |
uri |
http://data.open.ac.uk/oro/document/657903 |
| 56137 |
uri |
http://data.open.ac.uk/oro/document/657904 |
| 56137 |
uri |
http://data.open.ac.uk/oro/document/657905 |
| 56137 |
uri |
http://data.open.ac.uk/oro/document/657906 |
| 56137 |
uri |
http://data.open.ac.uk/oro/document/730340 |
| 56137 |
uri |
http://data.open.ac.uk/oro/document/815645 |
| 56137 |
volume |
133 |
| 56137 |
type |
AcademicArticle |
| 56137 |
type |
Article |
| 56137 |
label |
Wismayer, K.; Mehrban, N.; Bowen, J. and Birchall, M. (2019). Improving cellular
migration in tissue-engineered laryngeal scaffolds. Journal of Laryngology & Otology,
133(2) pp. 135–148. |
| 56137 |
label |
Wismayer, Kurt; Mehrban, Nazia; Bowen, James and Birchall, Martin A (2019). Improving
cellular migration in tissue-engineered laryngeal scaffolds. Journal of Laryngology
& Otology, 133(2) pp. 135–148. |
| 56137 |
Title |
Improving cellular migration in tissue-engineered laryngeal scaffolds |
| 56137 |
in dataset |
oro |