| 43263 |
Creator |
c3baa1aae2495d9fcf383c433b46bfe0 |
| 43263 |
Creator |
ext-c4eb8d1b7c4a66f90413226377b8158f |
| 43263 |
Creator |
ext-1310693160ef46f7204e54f75f772521 |
| 43263 |
Creator |
ext-273c59a3605ff5d3667abd0e6d01167b |
| 43263 |
Creator |
ext-3ff75ec9babdbb6d8cfb1294c72c5e90 |
| 43263 |
Date |
2014-12-13 |
| 43263 |
Is Part Of |
repository |
| 43263 |
abstract |
Cell surface proteins, for example specific antigen receptors on lymphocytes, have
become common targets for interrogation via atomic force microscopy (AFM). While the
majority of studies use live cells in order to mimic in vivo conditions, this is not
always feasible. For successful AFM adhesion measurements, cells that exhibit poor
natural adhesion to a counter-surface need to be immobilised, or have their mobility
restricted. To be able to interrogate structures within tissue sections – for example
germinal centres in spleen sections – fixation is required for preserving the structure
of the tissue for the long duration of the interrogation, as well as preserving any
applied immunohistochemical staining necessary for identification of regions of interest.
The work presented here investigates the effects that the commonly used fixatives
acetone and paraformaldehyde (PFA) have on biological samples, and their subsequent
influences on interactions between AFM tip and cell surface proteins in adhesion measurements. |
| 43263 |
authorList |
authors |
| 43263 |
presentedAt |
ext-d78c538da1453072fffe6eb00b772c8c |
| 43263 |
status |
nonPeerReviewed |
| 43263 |
type |
AcademicArticle |
| 43263 |
type |
Article |
| 43263 |
label |
Müller, Thomas; Marshall, Jennifer; Dehghani, Hamid; Bowen, James and Toellner, Kai-Michael
(2014). A sticky tale: sample preparation technique determines cell surface receptor
mobility and adhesion. In: 6th AFMBioMed Conference, 13-17 Dec 2014, San Diego,
USA. |
| 43263 |
label |
Müller, Thomas; Marshall, Jennifer; Dehghani, Hamid; Bowen, James and Toellner,
Kai-Michael (2014). A sticky tale: sample preparation technique determines cell surface
receptor mobility and adhesion. In: 6th AFMBioMed Conference, 13-17 Dec 2014, San
Diego, USA. |
| 43263 |
Title |
A sticky tale: sample preparation technique determines cell surface receptor mobility
and adhesion |
| 43263 |
in dataset |
oro |