Size-exclusion chromatography
Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. The chromatography column is packed with fine, porous beads which are commonly composed of dextran, agarose, or polyacrylamide polymers. The pore sizes of these beads are used to estimate the dimensions of macromolecules. SEC
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- YagoPermanentlyLocatedEntity
- Acronym
- enSEC
- Analytes
- enbiomolecules
- enmacromolecules
- ensynthetic polymers
- By
- enno
- Caption
- enEquipment for running size-exclusion chromatography. The buffer is pumped through the column by a computer-controlled device.
- Classification
- Chromatography
- Comment
- enSize-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. The chromatography column is packed with fine, porous beads which are commonly composed of dextran, agarose, or polyacrylamide polymers. The pore sizes of these beads are used to estimate the dimensions of macromolecules. SEC
- Depiction
- Has abstract
- enSize-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. The chromatography column is packed with fine, porous beads which are commonly composed of dextran, agarose, or polyacrylamide polymers. The pore sizes of these beads are used to estimate the dimensions of macromolecules. SEC is a widely used polymer characterization method because of its ability to provide good molar mass distribution (Mw) results for polymers.
- Hypernym
- Method
- Is primary topic of
- Size-exclusion chromatography
- Label
- enGel permeation chromatography
- Label
- enSize-exclusion chromatography
- Lcheading
- enGel permeation chromatography
- Link from a Wikipage to another Wikipage
- Adsorbent
- Agarose
- Aqueous normal-phase chromatography
- Aqueous solution
- Biomolecule
- Calibration curve
- Category:Biochemistry methods
- Category:Chromatography
- Category:Laboratory techniques
- Category:Molecular biology
- Category:Polymers
- Category:Polyolefins
- Chromatography
- Dextran
- Differential refractometer
- Dow Chemical Company
- Dynamic light scattering
- Elution
- File:Chrom SephG-50.tif
- File:SizeExChrom.png
- File:Size Exclusion Chromatography Apparatus.jpg
- File:Size exclusion standardisation.png
- File:Superdex 200HR gel filtration column.jpg
- Fractionation
- Friction
- Gel electrophoresis
- Gel permeation chromatography
- High-performance liquid chromatography
- Hydrodynamic radius
- Hydrodynamic volume
- Ion exchange chromatography
- Jerker Porath
- Logarithm
- Low-angle laser light scattering
- Macromolecule
- Micellar liquid chromatography
- Molar mass distribution
- Molecular weight
- Molecule
- Multi-Angle Laser Light Scattering
- Multi-angle light scattering
- Normal distribution
- PEGylation
- Per Flodin
- Polyacrylamide
- Polydispersity
- Polymer
- Polymer characterization
- Polymer fractionation
- Polystyrene
- Quaternary structure
- Radius of gyration
- Refractive index
- Solution (chemistry)
- Spectroscopy
- Static light scattering
- Synthetic polymer
- Tertiary structure
- THF
- Viscometry
- Void volume
- Manufacturers
- enCytiva, Bio-Rad, Bio-Works, emp Biotech, Knauer.
- Name
- enSize-exclusion chromatography
- Onlinebooks
- enno
- Related
- Aqueous normal-phase chromatography
- High-performance liquid chromatography
- Ion exchange chromatography
- Micellar liquid chromatography
- SameAs
- bhmk
- Chromatographie d'exclusion stérique
- Cromatografia di esclusione molecolare
- Eksklusioonkromatograafia
- Gel filtração
- Gelska izključitvena kromatografija
- m.0p5b
- Q1617865
- Q97377703
- Size exclusionchromatografie
- Size-exclusion chromatography
- Size-exclusion chromatography
- Витісна хроматографія
- Эксклюзионная хроматография
- کروماتوگرافی اندازهای
- 尺寸排除色谱法
- Subject
- Category:Biochemistry methods
- Category:Chromatography
- Category:Laboratory techniques
- Category:Molecular biology
- Category:Polymers
- Category:Polyolefins
- Thumbnail
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- Wikipage page ID
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- Wikipage revision ID
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- Template:Chromatography
- Template:Infobox chemical analysis
- Template:Library resources box
- Template:Protein structure determination
- Template:Reflist
- Template:Short description
