Chromogenic in situ hybridization
Chromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu oncogene amplification. CISH is similar to FISH in that they are both in situ hybridization techniques used to detect the presence or absence of specific regions of DNA. However, CISH is much more practical in diagnostic laboratories because it uses bright-field microscopes rather than the more expensive and complicated fluorescence microscopes used in FISH.
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- enChromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu oncogene amplification. CISH is similar to FISH in that they are both in situ hybridization techniques used to detect the presence or absence of specific regions of DNA. However, CISH is much more practical in diagnostic laboratories because it uses bright-field microscopes rather than the more expensive and complicated fluorescence microscopes used in FISH.
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- enChromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu oncogene amplification. CISH is similar to FISH in that they are both in situ hybridization techniques used to detect the presence or absence of specific regions of DNA. However, CISH is much more practical in diagnostic laboratories because it uses bright-field microscopes rather than the more expensive and complicated fluorescence microscopes used in FISH.
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- Chromogenic in situ hybridization
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- enChromogenic in situ hybridization
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- 2,4-Dinitrophenol
- Alkaline phosphatase
- Bacterial artificial chromosome
- Biotin
- Bovine serum albumin
- Category:Molecular biology techniques
- Digoxigenin
- File:CISH workflow.png
- File:FISH versus CISH Detection.png
- Fluorescein
- Fluorescein isothiocyanate
- Fluorescence in situ hybridization
- Horseradish peroxidase
- Human Genome Project
- Immunohistochemistry
- Monoclonal antibody
- Nick translation
- Polyclonal antibodies
- Streptavidin
- Trastuzumab
- SameAs
- 4hx7S
- Chromogenic in situ hybridization
- Hibridación in-situ cromogénica
- Hibridación in situ cromoxénica
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- Q5113886
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- Category:Molecular biology techniques
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