| 68686 |
Creator |
a5841b98ed42418276d2e88035d61953 |
| 68686 |
Creator |
25f5503348bcfe7d5ff29d613946ff35 |
| 68686 |
Creator |
ext-02a8f265769867b27d53ab6c81fa3c2a |
| 68686 |
Creator |
ext-37086d9fc35364c5445adbd46099c361 |
| 68686 |
Creator |
ext-3f5ff2442ef7daa6452b5b9aa2e1ea0f |
| 68686 |
Creator |
ext-53ef7e9b02f5ebd360ccae6e31abc300 |
| 68686 |
Creator |
ext-605482a6baadd1c968dc385fd70574b0 |
| 68686 |
Creator |
ext-7b1094c64d0f3e2f7a8b3deee80c3331 |
| 68686 |
Date |
2019-12-11 |
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Is Part Of |
repository |
| 68686 |
Is Part Of |
p20452322 |
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abstract |
The analysis of volatile organic compounds (VOCs) as a non-invasive method for disease
monitoring, such as type 2 diabetes (T2D) has shown potential over the years although
not yet set in clinical practice. Longitudinal studies to date are limited and the
understanding of the underlying VOC emission over the age is poorly understood. This
study investigated longitudinal changes in VOCs present in faecal headspace in two
mouse models of T2D - Cushing's syndrome and single <i>Afmid</i> knockout mice. Longitudinal
changes in bodyweight, blood glucose levels and plasma insulin concentration were
also reported. Faecal headspace analysis was carried out using selected ion flow tube
mass spectrometry (SIFT-MS) and thermal desorption coupled to gas chromatography-mass
spectrometry (TD-GC-MS). Multivariate data analysis of the VOC profile showed differences
mainly in acetic acid and butyric acid able to discriminate the groups <i>Afmid</i>
and Cushing's mice. Moreover, multivariate data analysis revealed statistically significant
differences in VOCs between Cushing's mice/wild-type (WT) littermates, mainly short-chain
fatty acids (SCFAs), ketones, and alcohols, and longitudinal differences mainly attributed
to methanol, ethanol and acetone. <i>Afmid</i> mice did not present statistically
significant differences in their volatile faecal metabolome when compared to their
respective WT littermates. The findings suggested that mice developed a diabetic phenotype
and that the altered VOC profile may imply a related change in gut microbiota, particularly
in Cushing's mice. Furthermore, this study provided major evidence of age-related
changes on the volatile profile of diabetic mice. |
| 68686 |
authorList |
authors |
| 68686 |
issue |
1 |
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status |
published |
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status |
peerReviewed |
| 68686 |
uri |
http://data.open.ac.uk/oro/document/1050443 |
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uri |
http://data.open.ac.uk/oro/document/1050444 |
| 68686 |
uri |
http://data.open.ac.uk/oro/document/1050445 |
| 68686 |
uri |
http://data.open.ac.uk/oro/document/1050446 |
| 68686 |
uri |
http://data.open.ac.uk/oro/document/1050447 |
| 68686 |
uri |
http://data.open.ac.uk/oro/document/1050448 |
| 68686 |
uri |
http://data.open.ac.uk/oro/document/1051244 |
| 68686 |
volume |
9 |
| 68686 |
type |
AcademicArticle |
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type |
Article |
| 68686 |
label |
Lourenco, Celia ; Kelly, Darren; Cantillon, Jack; Cauchi, Michael; Yon, Marianne A;
Bentley, Liz; Cox, Roger D and Turner, Claire (2019). Monitoring type 2 diabetes
from volatile faecal metabolome in Cushing's syndrome and single Afmid mouse models
via a longitudinal study. Scientific reports, 9(1), article no. 18779. |
| 68686 |
Title |
Monitoring type 2 diabetes from volatile faecal metabolome in Cushing's syndrome and
single <i>Afmid</i> mouse models via a longitudinal study |
| 68686 |
in dataset |
oro |