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Creator |
34150f2f89a55de66712a0576f106400 |
| 62326 |
Creator |
7c9060b8651fa54e11ffd46d3aba6da8 |
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Creator |
bd12e480200a0e58504d6106bfed3c8a |
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Creator |
0519c7e3d5b481315278655a3c8c63cf |
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Creator |
c893d03cffd868f18d028d07b803bfbe |
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Creator |
ext-899399f16734d9ff1df6e04278eafd6b |
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Creator |
ext-f00699cba616b8bf98a7c94a4e2d8247 |
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Date |
2018 |
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Is Part Of |
repository |
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abstract |
The aquifer fed springs of Colour Peak in the Canadian arctic are perennially cold,
highly saline and sulphur rich. These properties have led to the springs being considered
analogous to subsurface aqueous environments on Mars[1-3]. Therefore, understanding
the phylogenetic and functional diversity of prokaryotes that are capable of growing
in this environment is important for understanding the mechanisms conducive to life
within these extra-terrestrial environments.
Cultivation-dependant and -independent techniques were used to assess the diversity
of prokaryotes present in sediment collected from a hyper-saline cold pool at Colour
Peak. The microbial community that is present and active at the site was characterised
through the sequencing of 16S rRNA gene amplicons amplified following the extraction
of DNA and RNA from the sediment. To further investigate the metabolic profile of
the prokaryotes within the site, the presence and expression of functional genes relating
to the cycling of carbon, nitrogen and sulphur compounds was also assayed using PCR.
The results gained from the cultivation-independent work was used to inform enrichment
strategies, allowing for the isolation and characterisation of several halophilic
isolates. Through assessment of the physiologies of the isolates it was possible to
develop a further insight into the metabolic potential underpinning survival in Colour
Peak.
Through this work we have been able to develop an indication as to identity of the
key players and metabolic guilds within the microbial community of this site and further
our understanding of survival under this range of selection pressures. |
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authorList |
authors |
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presentedAt |
ext-7d861998d8f73c713dc12bc0af8c3a03 |
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status |
peerReviewed |
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type |
AcademicArticle |
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type |
Article |
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label |
Macey, Michael Christopher ; Fox-Powell, Mark; Stephens, Ben ; Schwenzer, Susanne
P. ; Pearson, Victoria K. ; Cousins, Claire R. and Olsson-Francis, Karen (2018).
Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour
Peak. In: Molecular Microbial Ecology Group (MMEG) Meeting 2018, 17-19 Dec 2018,
Swansea. |
| 62326 |
label |
Macey, Michael Christopher ; Fox-Powell, Mark; Stephens, Ben ; Schwenzer, Susanne
P. ; Pearson, Victoria K. ; Cousins, Claire R. and Olsson-Francis, Karen (2018).
Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour
Peak. In: Molecular Microbial Ecology Group (MMEG) Meeting 2018, 17-19 Dec 2018,
Swansea. |
| 62326 |
Title |
Arctic microbes – The phylogenetic and functional diversity of prokaryotes at Colour
Peak |
| 62326 |
in dataset |
oro |