| 36671 |
Creator |
3d9f5a6838060f791e133fa6e5265b06 |
| 36671 |
Creator |
ext-27f0b4d3c6faaf7a5d4f46d34aae59b1 |
| 36671 |
Creator |
ext-39767a58fc009ceccdd1c6c37f64a981 |
| 36671 |
Creator |
ext-a83de3d73b52b4c7ca258b007c954b89 |
| 36671 |
Creator |
ext-b9a3907b975ee2977a8c614445efaf59 |
| 36671 |
Date |
2013-02-08 |
| 36671 |
Is Part Of |
p14523981 |
| 36671 |
Is Part Of |
repository |
| 36671 |
abstract |
Electroactive biofilms are capable of extracellular electron transfer to insoluble
metal oxides and electrodes; such biofilms are relevant to biogeochemistry, bioremediation,
and bioelectricity production. We investigated the extracellular electron transfer
mechanisms in Shewanella loihica PV-4 viable biofilms grown at indium tin oxide (ITO)
and graphite electrodes in potentiostat-controlled electrochemical cells poised at
0.2 V vs. Ag/AgCl. Chronoamperometry and confocal microscopy showed higher biofilm
growth at graphite compared to the ITO electrode. Cyclic voltammetry, differential
pulse voltammetry, along with fluorescence spectroscopy showed that direct electron
transfer through outer membrane c type cytochromes (Omcs) prevailed at the biofilm/ITO
interface, while biofilms formed at graphite electrode reduced the electrode also
via secreted redox mediators, such as flavins and quinones. The biofilm age does not
affect the prevalent transfer mechanism at ITO electrodes. On the other hand, secreted
redox mediators accumulated at biofilm/graphite interface, thus increasing mediated
electron transfer as the biofilm grows over five days. Our results showed that the
electrode material determined the prevalent electron transfer mechanism and the dynamic
of secreted redox mediators in S. loihica PV-4 biofilms. These observations have implications
for the optimization of biofilm-based electrochemical systems, such as biosensors
and microbial fuel cells. |
| 36671 |
authorList |
authors |
| 36671 |
status |
peerReviewed |
| 36671 |
uri |
http://data.open.ac.uk/oro/document/132622 |
| 36671 |
uri |
http://data.open.ac.uk/oro/document/132638 |
| 36671 |
uri |
http://data.open.ac.uk/oro/document/132640 |
| 36671 |
uri |
http://data.open.ac.uk/oro/document/132641 |
| 36671 |
uri |
http://data.open.ac.uk/oro/document/132642 |
| 36671 |
uri |
http://data.open.ac.uk/oro/document/132643 |
| 36671 |
uri |
http://data.open.ac.uk/oro/document/133172 |
| 36671 |
volume |
8 |
| 36671 |
type |
AcademicArticle |
| 36671 |
type |
Article |
| 36671 |
label |
Jain, Anand; O Connolly, Jack; Woolley, Richard; Krishnamurthy, Satheesh and Marsili,
Enrico (2013). Extracellular electron transfer mechanism in Shewanella loihica PV-
4 biofilms formed at indium tin oxide and graphite electrodes. International Journal
of Electrochemical Science, 8 pp. 1778–1793. |
| 36671 |
label |
Jain, Anand; O Connolly, Jack; Woolley, Richard; Krishnamurthy, Satheesh and Marsili,
Enrico (2013). Extracellular electron transfer mechanism in Shewanella loihica PV-
4 biofilms formed at indium tin oxide and graphite electrodes. International Journal
of Electrochemical Science, 8 pp. 1778–1793. |
| 36671 |
Title |
Extracellular electron transfer mechanism in Shewanella loihica PV- 4 biofilms formed
at indium tin oxide and graphite electrodes |
| 36671 |
in dataset |
oro |