| 171180 |
url |
holdingsInfo?bibId=171180 |
| 171180 |
isAbout |
model |
| 171180 |
isAbout |
amino acids |
| 171180 |
isAbout |
enzyme kinetics |
| 171180 |
isAbout |
carrier mediated transport |
| 171180 |
isAbout |
maximal velocity |
| 171180 |
isAbout |
michaelis constant |
| 171180 |
Contributor |
287a63ebe51883cabf1c719922e2e1bc |
| 171180 |
Table Of Contents |
Vic Daniels introduces the programme which will discuss the basic approach to enzyme
kinetics using carrier mediated transport. Daniels uses a model of an enzyme molecule
to demonstrate its action as a carrier for a substrate. He compares this process with
an enzyme reaction where a product is formed. Daniels discusses rate of reaction
for the enzyme-substrate carrier process. He uses the model to aid. Animated diagram
of a cross-section of a membrane shows the carrier reaction. The diagram shows the
relationship between amount of substrate and velocity of the carrier reaction. A graph
shows the relationship between amount of substrate and reaction velocity. Daniels
explains how the derivation of the kinetic constants can be simplified. A reciprocal
curve form is plotted on the graph and a straight line curve results. Daniels uses
the enzyme model again to explain what happens when a reaction inhibitor is introduced.
He discusses "competivive inhibition" first. This reaction is plotted on a graph.
Daniels explains "non-competitive reactions". He continues to use the enzyme model
to aid his commentary. This reaction is also shown on the graph. Daniels sums up his
discussion of competitive and non-competitive reactions in the enzyme carrier process.
Daniels introduces Paul Voorheis. Paul Voorheis discusses his work: on the transport
kinetics of trypanosomes. Shots of trypanosomes under magnification (they are the
cause of African Sleeping Sickness) Voorheis discusses his choice of trypanosomes
for amino acid transport experiments. Voorheis demonstrates the purification process
which obtains samples of trypanosomes from rat blood. The blood is centrifuged and
then further separated by column chromatography Both processes are explained and chromatography
is demonstrated. Voorheis adds the isolated trypanosomes to a solution of radio-active
amino acid. Transport rate is measured in a scintillation counter. Voorheis explains
the experiment each step of the way. The count is displayed on the instrument. Voorheis
uses several graphs to plot the curves for the effect of substrate concentration
on uptake of amino acid. Voorheis explains the effect of introducing an inhibitor
to two carriers and shows a graph which plots the results. He explains why it is difficult
to explain the curves in terms of their kinetic constants. Voorheis explains how
this problem is overcome by using a computer. Shots of a chart showing computer
generated data of the effect of inhibitors on kinetic constants. Voorheis sums up.
He describes future experiments which may be done to determine why trypanosomes have
two carriers for the same amino acid. |
| 171180 |
locator |
holdingsInfo?bibId=171180 |
| 171180 |
type |
Collection |
| 171180 |
label |
Transport kinetics |
| 171180 |
Description |
Programme examines the basic approach to enzyme kinetics using carrier mediated transport. |
| 171180 |
Publisher |
07f21de77e80d6bd38c3be6db6cb323d |
| 171180 |
Title |
Transport kinetics |
| s321 |
has-courseware |
171180 |