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has-courseware |
170358 |
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url |
holdingsInfo?bibId=170358 |
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P1007 |
produced by Roger Jones; contributor David Phillips. |
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isAbout |
model |
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isAbout |
3-d structure |
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isAbout |
computer animations |
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isAbout |
lysozyme |
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isAbout |
video animations |
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Contributor |
3fadbf8f4d96564844175155a5a8fa47 |
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Contributor |
e338e1a574faaa0020cfd7bbf8cff14f |
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Table Of Contents |
Film, taken at 2000 magnifications, of bacterial cells being broken down by the enzyme
lysozyme. Commentary by David Phillips. Shots of an atomic model of lysozyme showing
its 129 amisso acid residues spread out in one long line. David Phillips walks alongside
the model and points out some of the individual residues. Shots of a high resolution
atomic model of lysozyme folded into its actual globular structure. Phillips explains
how electron density maps are built up for a molecule like lysozyme by the technique
of X-ray diffraction. Shots of the equipment with an explanation by Phillips of how
it works. Shots of electron density maps. Phillips goes on to explain how it is possible
to determine which contours on the electron density map represent which residues in
the molecule. Electron density maps, atomic models and computer animations help illustrate
his points. Phillips next demonstrates a more sophisticated method for fitting a molecule
to an electron density map. This method, developed at Leeds University, uses a computer
to calculate contours for a single value of election density for cross sections in
three directions. Computer animations show the result. Commentary by Phillips. A computer
animation builds up a 3 dimensional image of the lysozyme molecule. For clarity, side
chains are not shown. Most of the residues are coloured red but those which make up
the three helices are coloured green. Shots of a low resolution atomic model superimposed
over a high resolution model of lysozyme in order that the structure may be seen more
clearly. While Phillips sums up, shots of the atomic model of lysozyme viewed from
above to reveal a distinct cleft in the globular shape. |
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locator |
holdingsInfo?bibId=170358 |
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type |
Collection |
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label |
The structure of lysozyme |
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Description |
This programme describes the techniques which were used to determine the structure
of lysozyme and emphasises certain distinctive features of the molecule. |
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Publisher |
07f21de77e80d6bd38c3be6db6cb323d |
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Title |
The structure of lysozyme |